Module 5 Instructor Resources¶
Lesson Plan¶
Title¶
- Translation: The need for an Open Reading Frame
Objectives¶
- Determine the codons for specific amino acids and identify reading frames by looking at the Base Position track in the genome browser
- Assemble exons to maintain the open reading frame (ORF) for a given gene
- Define the phases of the splice donor and acceptor sites and describe how they impact the maintenance of the ORF
- Identify start and stop codons of an assembled ORF
Pre-requisites¶
Order¶
- Warm Up/Review of Pre-requisites
- Investigation 1
- Investigation 2
- Exit
Homework¶
- None
Class Instruction¶
- Review the process of translation: Overview of the ribosome, tRNAs, and associated proteins involved in translation (Initiation Factors, Elongation Factors and Release Factors)
- Review the DNA codon table
- Work through the activities using the Genome Browser, with pauses to discuss the answers to the questions.
- Conclude with emphasis on main points:
- mRNAs are translated into amino acids using triplet codons
- Identification of ORFs
- The ORF must be maintained across splice sites to generate a working mRNA
- The assembled ORF begins with a start codon and ends with a stop codon.