Module 4 Instructor Resources¶
Lesson Plan¶
Title¶
- Removal of introns from messenger RNA by splicing
Objectives¶
- Identify splice donor and acceptor sites that are best supported by RNA-Seq and TopHat splice junction predictions
- Utilize the canonical splice donor and splice acceptor sequences to identify intron-exon boundaries.
Order¶
- Review mRNA processing; Investigation 1
- Define isoform
- Introduce consensus sequences; Investigation 2
- Discuss pre-mRNA vs. mRNA
- Explore how to find exon-intron junction using tra-RA as the example; Investigation 3
Homework¶
- Students will identify intron-exon junctions for spd-2-RA
Class Instruction¶
- Review pre-mRNA processing using appropriate figures from the textbook or Module 3
- Investigation 1: Students familiarize themselves with RNA-Seq data
- Review consensus sequences for splice donor and splice acceptor sites
- Investigation 2: Students find splice donor and acceptor for intron 1
- Review RNA splicing of intron 1 using tra-RA as the example
- Investigation 3: Students find remaining splice donor and splice acceptor for intron 2
- Discuss length of pre-mRNA vs. length of spliced mRNA
- Identify isoforms with different TSSs, or alternative splicing patterns